Biological samples require a hydrated environment to maintain their structure and retain dynamic behavior. With Protochips’ machine-vision powered suite of in-situ TEM tools you can utilize the electron microscope as a real-time laboratory to study delicate, low contrast systems at a resolution of a few nanometers.
Study nanoscale dynamics, interactions, and processes of virus, cells, proteins, micelles, and other biological and biomimetic materials while maintaining a physiologically relevant environment.Click here to learn more about our liquid cell solution
In this application note, written by Protochips, we summarize how in situ electron microscopy using liquid phase is challenged by radiolysis side effects and how to minimize these effects.
In this paper, we take a look at how AXON Dose tracks the electron flux and cumulative dose, and why this might be useful for all experiments.
This paper, written by Protochips, summarizes a collection of published literature on nanoparticle movement within living cells using the Poseidon system.
In this summarized paper, Dr. Diana B. Peckys was able to image live yeast cells in hydrated form in the electron microscope using the Poseidon system.
In this summarized paper, researchers from the group of Dr. Niels de Jonge cultivated COS -7 fibroblast cells directly on Poseidon E-chips.
In this summarized paper, rotavirus particles were imaged by using affinity capture in solution using a Poseidon in situ liquid TEM holder and then 3D constructed.
In this summarized paper, Deborah Kelly’s group at the Virginia Tech Carilion Research Institute in Roanoke, VA successfully imaged pegylated liposomal control vehicles, under low dose conditions using the Poseidon system.
In this summarized paper, researchers from the group of Dr. Alloyeau looked at the stability of carbon nanotubes in different liquids to understand the degradation of these materials in macrophage cells.
Watch dynamic behavior of real samples in situ.
This movie shows a series of images that demonstrates the tilt range of Poseidon Select. Images of aggregated 30 nm gold nanoparticles in 1.5 μm of water were collected in 5° increments, over a total angle of -30° to +30°. Data was collected using a Philips/FEI CM300FEG TEM equipped with a GIF energy filter at 300 kV.
Electron beam-induced growth of calcite nanoparticles in situ in the presence of AP7, a nacre protein that is found in abalone shellfish. The calcite particles were grown in the spatially confined conditions of the in-situ liquid cell in a liquid layer of 500 nm. During growth both a stable protein mediated scaffold-type structure, and an unstable crystalline structure which re-dissolved shortly after formation, were observed. (JEOL JEM-2200 FS with double Cs correction, operated at 200 KV in STEM mode. Movie playback is increased by a factor of 25). Courtesy of Andreas Verch and Roland Kroeger. For more information on Poseidon Select, go to our website at http://www.protochips.com/products/poseidon.html
Learn about Protochips' groundbreaking liquid cell for in situ TEM and STEM.
AXON Synchronicity enables live drift correction of vesicular structures in fabric softener. Data was acquired using a Gatan 626 cryogenic holder on a Tecnai G2 Twin at the Shared Materials Instrumentation Facility at Duke University. This movie demonstrates the power of AXON’s image stabilization even at cryogenic temperatures with a non-Protochips holder. For more information about AXON Synchronicity, visit us at https://www.protochips.com/solutions/tem-data-correlation/tem-software/.
Additional information: movie was roughly 10min and acceleration voltage was 200kV