AXON Dose Publication Alert in Jove!
September 5, 2023
Biological samples require a hydrated environment to maintain their structure and retain dynamic behavior. With Protochips’ machine-vision powered suite of in-situ TEM tools you can utilize the electron microscope as a real-time laboratory to study delicate, low contrast systems at a resolution of a few nanometers.
Poseidon AX: Liquid, electrochemical and liquid heating in situ studies
Study nanoscale dynamics, interactions, and processes of virus, cells, proteins, micelles, and other biological and biomimetic materials while maintaining a physiologically relevant.
Click here to learn more about our liquid cell solution
Application Notes
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Studying Cells with Correlative Microscopy
Correlative light and electron microscopy of eukaryotic cells in liquid
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Yeast Cells in Liquid
Imaging live yeast cells without freezing or plastic embedment
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Atomic Resolution Imaging in Liquid
Imaging crystal lattice of coated gold nanorods in liquid
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3D Reconstruction of Rotovirus
Visualizing viral assemblies using liquid cell EM
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Visualizing Drug Delivery
Imaging liposomal control vehicles for advanced drug delivery applications
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Using Nanoparticles for Cancer Treatment
Improving cancer treatments by directly imaging nanoparticle movement within Gliobastoma cells
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Nanotube Interaction within Cells
Imaging the degradation of carbon nanotubes within macrophage cells
Videos
Watch dynamic behavior of real samples in situ.
Electron beam-induced growth of calcite nanoparticles in situ in the presence of AP7, a nacre protein that is found in abalone shellfish. The calcite particles were grown in the spatially confined conditions of the in-situ liquid cell in a liquid layer of 500 nm. During growth both a stable protein mediated scaffold-type structure, and an unstable crystalline structure which re-dissolved shortly after formation, were observed. (JEOL JEM-2200 FS with double Cs correction, operated at 200 KV in STEM mode. Movie playback is increased by a factor of 25). Courtesy of Andreas Verch and Roland Kroeger. For more information on Poseidon Select, go to our website at http://www.protochips.com/products/poseidon.html
This movie shows a series of images that demonstrates the tilt range of Poseidon Select. Images of aggregated 30 nm gold nanoparticles in 1.5 μm of water were collected in 5° increments, over a total angle of -30° to +30°. Data was collected using a Philips/FEI CM300FEG TEM equipped with a GIF energy filter at 300 kV.