Poseidon Select System Media

The Poseidon platform enables observation of dynamic processes in situ. This movie shows the formation of salt crystals from a saturated solution of phosphate buffered saline. Images were acquired at a rate of 1 frame per second and are shown at 5 times real speed. 500 nm liquid thickness. Images collected using a Philips/FEI CM300FEG TEM at 300 kV. Courtesy of Dr. Kate Klein, National Institute of Standards and Technology. For more information on Poseidon, visit http://bit.ly/protochipsposeidon.

This movie shows a series of images that demonstrates the tilt range of Poseidon Select Liquid. Images of aggregated 30 nm gold nanoparticles in 1.5 μm of water were collected in 5° increments, over a total angle of -30° to +30°. Data was collected using a Philips/FEI CM300FEG TEM equipped with a GIF energy filter at 300 kV. For more information on Poseidon Select, visit http://www.protochips.com/products/poseidon.html.

Electron beam-induced growth of calcite nanoparticles in situ in the presence of AP7, a nacre protein that is found in abalone shellfish. The calcite particles were grown in the spatially confined conditions of the in-situ liquid cell in a liquid layer of 500 nm. During growth both a stable protein mediated scaffold-type structure, and an unstable crystalline structure which re-dissolved shortly after formation, were observed. (JEOL JEM-2200 FS with double Cs correction, operated at 200 KV in STEM mode. Movie playback is increased by a factor of 25). Courtesy of Andreas Verch and Roland Kroeger. For more information on Poseidon Select, go to our website at http://www.protochips.com/products/poseidon.html

A mixture of spherical gold nanoparticles and gold nanorods suspended in water was imaged using the Protochips’ Poseidon Select Liquid in situ holder. The gold nanoparticles are highly mobile and can be observed moving and linking together over time. A 150 nm spacer configuration was used and the imaging acceleration voltage was 200 kV. For more information on Poseidon Select, visit http://bit.ly/protochipsposeidon.

30 nm gold nanoparticles attracted to the electron beam in a liquid thickness of 150 nm. Images collected using a Philips/FEI CM300FEG TEM at 300 kV. Courtesy of Dr. Kate Klein, National Institute of Standards and Technology. For more information on Poseidon, visit http://bit.ly/protochipsposeidon.

Electron beam induced growth of lead nanoparticles from a 40 mM aqueous solution of Pb(NO3)2. Nucleation was induced by the electron beam, and the particles grow via an Oswaldt ripening process followed by second growth stage in which the particles increase uniformly in size. The Oswaldt ripening process is indicated by the red circle. When these nanoparticles are in close proximity to larger nanoparticles, they decrease in size and ultimately disappear, while larger nanoparticles increase in size.

The movie was recorded using FEI Tecnai Biotwin operated at 120 KV and is courtesy of Dr. Albert D. Dukes, III, Lander University and Dr. Deborah Kelly, Virginia Tech.

Learn about Protochips' groundbreaking liquid cell for in situ TEM and STEM.

Learn how liquid cell EM is transforming the way researchers study the complex interactions in biological samples in their native, hydrated state.

Learn how leading scientists in battery research and functional materials are utilizing liquid cell EM to accelerate their research and uncover new findings in their lab.

Learn how liquid cell EM is transforming the way researchers study the complex interactions in biological samples in their native, hydrated state.

Learn how leading scientists in battery research and functional materials are utilizing liquid cell EM to accelerate their research and uncover new findings in their lab.