Currently, there remains a critical need to develop real-time imaging resources for life sciences. Here, we demonstrate the use of high resolution in situ imaging to observe biological complexes in liquid at the nanoscale. Using a model virus system, we produced the first time-resolved videos of individual biological complexes moving in solution within an electron microscope.
In situ visualization of RNA movement inside an actively transcribing rotavirus imaged using LC-TEM. The magnitude of dynamic movement within the electron dense core of the rotavirus increases with increasing transcriptional activity of the virus.