Visualizing virus particle mobility in liquid at the nanoscale

A. Cameron Varano, Amina Rahimi, Madeline J. Dukes, Steven Poelzing, Sarah M., 2015
rotavirus observied in situ via LC-TEM
Image courtesy of Chem Comm


Currently, there remains a critical need to develop real-time imaging resources for life sciences. Here, we demonstrate the use of high resolution in situ imaging to observe biological complexes in liquid at the nanoscale. Using a model virus system, we produced the first time-resolved videos of individual biological complexes moving in solution within an electron microscope.

Impact Statement

In situ visualization of RNA movement inside an actively transcribing rotavirus imaged using LC-TEM. The magnitude of dynamic movement within the electron dense core of the rotavirus increases with increasing transcriptional activity of the virus.