Imaging the Hydrated Microbe-Metal Interface Using Nanoscale Spectrum Imaging

Edward A. Lewis, Helen Downie, Richard F. Collins, Eric Prestat, Jonathan R Lloyd and Sarah J. Haigh, 2016

Abstract

PdAu nanocrystals are synthesised by Geobacter sulfurreducens, a dissimilatory metal-reducing bacterium, and the resulting bimetallic nanocrystal-decorated microbes are imaged using a range of advanced electron microscopy techniques. Specifically, the first example of elemental mapping of fully hydrated biological nanostructures using scanning transmission electron microscope (STEM) energy dispersive X-ray (EDX) spectrum imaging within an environmental liquid-cell is reported. These results are combined with cryo-TEM and ex situ STEM imaging and EDX analysis with the aim of better understanding microbial synthesis of bimetallic nanoparticles. It is demonstrated that although Au and Pd are colocalized across the cells, the population of nanoparticles produced is bimodal, containing ultrasmall alloyed nanocrystals with diameters <3 nm and significantly larger core-shell structures (>200 nm in diameter) which show higher Pd contents and exhibit a Pd enriched shell only a few nanometers thick. The application of high-resolution imaging techniques described here offers the potential to visualize the microbe-metal interface during the bioproduction of a range of functional materials by microbial “green” synthesis routes, and also key interfaces underpinning globally relevant environmental processes (e.g., metal cycling).

Impact Statement

Intact bacteria cells that synthesize metallic nanoparticles from precursers in their environment were imaged in situ using LC-STEM. EDS maps of the bacteria revealed showed the elemental make-up ofAuPd core-shell nanoparticles both within and on the surface of the bacteria.
Keywords: Cells; Nanoparticles; EDS