Studying delicate, organic samples typically requires analytical tools like X-ray crystallography, Cryo-EM, and NMR that only provide “snapshots” of real interactions. Using liquid cell EM, you can image biological samples in their native, hydrated environment at resolutions that allow you understand structural and chemical evolution in real-time.
Application Notes
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Studying Cells with Correlative Microscopy
Correlative light and electron microscopy of eukaryotic cells in liquid
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Yeast Cells in Liquid
Imaging live yeast cells without freezing or plastic embedment
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Atomic Resolution Imaging in Liquid
Imaging crystal lattice of coated gold nanorods in liquid
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3D Reconstruction of Rotovirus
Visualizing viral assemblies using liquid cell EM
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Visualizing Drug Delivery
Imaging liposomal control vehicles for advanced drug delivery applications
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Using Nanoparticles for Cancer Treatment
Improving cancer treatments by directly imaging nanoparticle movement within Gliobastoma cells
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Nanotube Interaction within Cells
Imaging the degradation of carbon nanotubes within macrophage cells
Videos
Watch dynamic behavior of real samples in situ.
Electron beam-induced growth of calcite nanoparticles in situ in the presence of AP7, a nacre protein that is found in abalone shellfish. The calcite particles were grown in the spatially confined conditions of the in-situ liquid cell in a liquid layer of 500 nm. During growth both a stable protein mediated scaffold-type structure, and an unstable crystalline structure which re-dissolved shortly after formation, were observed. (JEOL JEM-2200 FS with double Cs correction, operated at 200 KV in STEM mode. Movie playback is increased by a factor of 25). Courtesy of Andreas Verch and Roland Kroeger. For more information on Poseidon Select, go to our website at http://www.protochips.com/products/poseidon.html
This movie shows a series of images that demonstrates the tilt range of Poseidon Select. Images of aggregated 30 nm gold nanoparticles in 1.5 μm of water were collected in 5° increments, over a total angle of -30° to +30°. Data was collected using a Philips/FEI CM300FEG TEM equipped with a GIF energy filter at 300 kV.
Poseidon Select Liquid™ In Situ Cell: Protein Mediated Calcite Formation
Poseidon Select Tilt
