|Title||Authors||Journal Info||Impact Statement|
|In Situ Molecular Microscopy of Viral Assemblies
||Madeline J. Dukes, Brian L. Gilmore, Justin R. Tanner, Sarah M. McDonald and Deborah F. Kelly
||Method paper and corresponding video demonstrating step-by-step instructions for preparing "affinity-capture" functionalized Poseidon E-chips, capturing target species and imaging them in situ in liquid with TEM.
|Visualizing Nanoparticle Mobility in Liquid at Atomic Resolution
||Madeline J. Dukes, Benjamin W. Jacobs, David G. Morgan, Harshad Hegde and Deborah F. Kelly
||Chemical Communications DOI:10.1039/c0xx00000x
||The first high-resolution in-situ live video imaging the of the mobility of polyvinyl pyridine (PVP)- passivated gold nanorods and how they migrate in liquid.
|Visualizing viral assemblies in a nanoscale biosphere
||Brian L. Gilmore, Shannon P. Showalter, Madeline J. Dukes, Justin R. Tanner, Andrew C. Demmert, Sarah M. McDonald and Deborah F. Kelly
||Lab on a Chip, 13, pp 216-219, 2013
||First example of 3D reconstruction of a biological particle from liquid in situ TEM images. A 25 Å reconstruction was obtained from 600 double layer rotovirus particles imaged in a 150 nm liquid layer. Multiple subpopulations were identified, several of which, are not observed in cryo-prepared samples indicating the dynamic state of samples in liquid versus those embedded in ice.
|The development of affinity capture devices - a nanoscale purification platform for biological in situ transmission electron microscopy
||Katherine Degen, Madeline Dukes, Justin R. Tanner, Deborah F. Kelly
||RSC Advances, 2(6), pp 2408-2412, 2012
||First example of using "affinity-capture" surface modification of E-chips for tethering biological species to the E-chip surface for in situ liquid TEM imaging. Ribosome proteins expressing His-tags were captured in-situ onto Ni-NTA functionalized E-chips and imaged in 150 nm liquid using TEM.
|UV-Induced Photochemical Transformations of Citrate-capped Silver Nanoparticle Suspensions
||Justin M. Gorham, Robert I. MacCuspie, Kate L. Klein, D. Howard Fairbrother, R. David Holbrook
||Journal of Nanoparticle Research, 14(10), pp 1-16, 2012
||Poseidon was used to image the structure of silver nanoparticles in water after exposure to ultraviolet light under aqueaus conditions. Exposure to UV-light in an aqueaus environment resulted in a decrease in the size fo the particles and and the formation of a core–shell structure. This experiment shows the utilization of in-situ liquid TEM techniques for studying the environmental impact of nanomaterials.
|Video-frequency scanning transmission electron microscopy of moving gold nanoparticles in liquid
||Ring, E.A. & de Jonge, N.
||Micron, 43, pp 1078-1084, 2012
||Use of the E-chips to image gold nanoparticle movement; much slower movement was observed than what was expected on the basis of Brownian motion.
|Visualizing Gold Nanoparticle Uptake in Live Cells with Liquid Scanning Transmission Electron Microscopy
||D.B. Peckys, G.M. Veith, D.C. Joy and N. de Jonge
||Nano Lett., 11 (4), pp 1733–1738, 2011
||The uptake of 30 nm diameter gold nanoparticles into intracellular vesicles was imaged in-situ with STEM using Poseidon. The images were quantitatively analyzed to determine the particle number, loading percentage, and clustering behavior after 24 hours.
|Fully Hydrated Yeast Cells Imaged with Electron Microscopy
||N. de Jonge, D.B. Peckys, G.M. Veith, S. Mick, S. Pennycook and D. Joy
||Biophysical Journal, 100(10), pp 2522-2529, 2011
||Correlative fluorescence microscopy and scanning transmission electron microscopy of whole yeast cells in liquid. Multiple strains of S. Pombe yeast cells, that express different structural mutations, were imaged live in 3 µm of liquid using STEM. A resolution of 32 nm was obtained - without the addition of stain or nanoparticle labels. The yeast cells were alive at the onset of electron microscopy as evidenced by the fluorescence of a live-dead indictor fluorescent dye.
|Silicon nitride windows for electron microscopy of whole cells
||E.A. Ring, D.B. Peckys, M.J. Dukes, J.P. Baudoin N. de Jonge
||Journal of Microscopy, 243(3), pp 273-283, 2011
||Review of basic techniques for preparing whole cells for in-situ liquid imaging. Particular attention is given to the process of preparing the E-chips for tissue culture and culturing whole cells on the E-chips. Additional dry (non-liquid) applications which can be prepared in parallel to the in-situ samples are also discussed.
|Simulating STEM Imaging of Nanoparticles in Micrometers-Thick Substrates
||H. Demers, N. Poirier-Demers, Dominic Drouin, N. de Jonge
||Microscopy and Microanalysis, 16(6), pp 795-804, 2010
||Simulation of imaging in-situ using STEM. Monte Carlo simulations are performed to determine resolution, signal-to-noise ratio, beam broadening effect and electron scattering behavior. The simulations were compared to results obtained experimentally and were found to be similar.
|Electron Microscopy of Specimens in Liquid
||Niels de Jonge, Frances M. Ross
||Nature Nanotechnology, 6, pp 695-704, 2011
||Review paper focusing on recent advances of in-situ liquid electron microscopy.
|Corrrelative Fluorescence Microscopy and Scanning Transmission Electron Microscopy of Quantum-Dot-Labeled Proteins in Whole Cells in Liquid
||Madeline J. Dukes, Diana B. Peckys, Niels de Jonge
||ACS Nano, 4, pp 4110-4116, 2010
||Correlative light and electron microscopy of whole cells labeled with quantum dots. Epidermal growth factor receptors on COS7 fibroblast cells were labeled with red-emitting EGF-quantum dots and imaged with wide field microscopy. The same regions were relocated in the electron microscope and STEM images of the individual quantum dot nanoparticles were recorded.
|Atmospheric Pressure Scanning Transmission Electron Microscopy
||Niels de Jonge, Wilbur C. Bigelow, Gabriel M. Veith
||Nano Letters, 10, pp 1028-2031, 2010
||STEM imaging of gold nanoparticles at atmospheric pressure in a mixture of CO, O2, and He. Silicon Nitride membranes were used to create a fluidic cell for gas containment.
|Nanometer-Resolution Electron Microscopy Through Micrometers-Thick Water Layers
||N. de Jonge, N. Poirier-Demers, H. Demers, D.B. Peckys, and D. Drouin
||Ultramicroscopy, vol. 110, pp. 1114-1119, 2010
||Experimental and theoretical comparison of resolution, SNR, and imaging conditions using in situ liquid STEM to image gold nanoparticles in a variety of liquid thicknesses.
|Microfluidic System for Transmission Electron Microscopy
||Elisabeth A. Ring, Niels de Jonge
||Microscopy and Microanalysis, vol 16, pp 622-629, 2010
||Flow rates and behavior through a liquid chamber formed using Poseidon E-chips were investigated. The flow was investigated optically using quantum dots with wide-field fluorescence microscopy and gold nanoparticles were utilized for in-situ liquid TEM measurements. The rate which the nanoparticles moved through the chamber with respect to the pump speed was determined while considering influences such as Brownian motion and bypass channel depth.
|Transmission electron microscopy with a liquid flow cell
||Klein, K.L., Anderson, I.M. & de Jonge, N.
||Journal of Microscopy, 242(2), pp 117-123, 2010
||Demonstrated that the Poseidon system can be used both for TEM and STEM.
|Electron Microscopy of Whole Cells in Liquid With Nanometer Resolution
||N. de Jonge, D.B. Peckys, G.J. Kremers, D.W. Piston
||PNAS, 106, pp 2159-2164, 2009
||Whole cells imaged in liquid using in situ STEM. Chemically fixed COSy cells were labeled with EGF-targeted gold nanoparticles and imaged using STEM. Describes STEM beam scattering experimentally and theoretically. Compares results obtained in the STEM to optical microscopy, SEM and normal TEM operation. Uses Protochips E-chips with a Hummingbird holder.
|Nanoscale Imaging of Whole Cells Using a Liquid Enclosure and a Scanning Transmission Electron Microscope
||Diana B. Peckys, Gabriel M. Veith, David C. Joy, Niels de Jonge
||PLoS One, 4, pp e8214, 2009
||Describes sample prep methods, sample loading and construction of the E-chips. Imaging epidermal growth factor (EGF) receptors on mammalian cells and E coli using gold nanoparticles as markers. Found that 50 nm thick silicon nitride membranes were optimal for electron transparency and physical strength. Discusses dose and radiation damage in cells. 1 x 10^4 e/nm2 is observed to be a threshold for damage.