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Application Notes

Aduro Application Notes
AA01.2	Correlative Analysis in the TEM and SEM	The Kirkendall effect was imaged and analyzed using the TEM and SEM. Results were correlated to garner understanding of dynamic processes.
AA02.2	Melting and Sinter Behavior of Silver Nanoparticles in the TEM	Silver nanoparticles were analyzed to determine melting and vaporization temperatures and sintering characteristics as a function of size.
AA03.2	In Situ Measurements of the Thermal Properties of Carbon Nanotube/Silver Nanowires	Two in situ experiments were preformed to determine the thermal properties of carbon nanotube/silver nanowires.
AA04.2	Thermal Drift and Settle Times measured for Different Temperature Excursions in the TEM	Thermal drift and settle time characteristics of the Aduro platform were quantified with two experiments.
AA05.2	EDS in the TEM and SEM with Aduro	The Aduro provides the clear line of sight necessary for EDS compatibility. Elemental maps were collected from two samples.
AA06.2	Ion Beam Deposition of Tungsten Metal onto Thin Silicon Nitride Membranes	Ion beam deposition of tungsten metal onto thin silicon nitride membranes. Four point probe use to measure resistance of deposited tungsten.
AA07.2	PdZn Alloy Nanoparticles on ZnO Nanobelts	Using the Aduro platform PdZn was analyzed at the atomic scale to better understand the formation processes of these alloys.
AA08.2	Visualizing Temperature Evolution of Metal Nanoparticle Catalysts in High Resolution	Gold nanoparticles on iron oxide were heated up to 700 °C and dynamically observed through the water-gas shift and the CO oxidation reaction.
AA09.2	Thermal Stability Study of LAST Thermoelectric Material in the SEM	Lead Antimony Silver telluride was heated to observe how the elemental components evolved as a function of temperature.
AA10.2	Atomic Resolution Imaging at High Temperatures in the TEAM 0.5	Au nanoparticles were imaged at 600 °C with a resolution of .7 Angstroms in the TEAM .5 TEM.
AA11.2	In Situ FCC to L10 Tetragonal Phase Transition of Magnetic FePt Nanoparticles	The formation of the fcc to L10 phase transition was observed using the Aduro platform.

Aduro Sample Preparation
FIB Lamella	Prepare integrated circuits, solar cells, batteries and magnetic on Thermal and Electrical E-chips using FIB methods.
Nanowires	Three possible methods are detailed to transfer nanowires from their growth substrate to the thin membrane on an Aduro E-chip.
Particles	Detailed instructions for the wet and dry deposition of samples on Aduro E-chips.

Poseidon Application Notes
AP30.1	Correlative Light and Electron Microscopy (CLEM) of Eukaryotic Cells in Liquid	Wet samples were imaged utilizing the CLEM imaging strategy eliminating the need for drying or freezing samples.
AP31.1	Imaging Gold Nanoparticles of Different Sizes in Liquid Using TEM	Aqueous gold nanoparticles were imaged with the Poseidon. Wet imaging unlocks the mechanisms that control dynamic processes such as nucleation, growth, and self-assembly.
AP32.1	Scanning Electron Microscopy of Live Yeast Cells in Liquid	Live Yeast cells were imaged with an STEM without freezing or plastic embedment.
AP40.1	Imaging Nanoparticle Flow With Scanning Transmission Electron Microscopy	The Poseidon™ GapSet™ E-chip™ design is ideal for imaging a continuous flow of liquid and for the injection of reagents into the system.
AP41.1	Flow Characterization of Poseidon™ GapSet™ E-chips™	The GapSet™ E-chip™ channel design permits rapid exchange (~1 minute), of liquid within the sample chamber.
AP42.1	Polyvinyl Pyridine Coated Gold Nanorods Imaged in Liquid	Gold nanorods encapsulated in a layer of polyvinyl pyridine(PVP) were imaged in a 150 nm layer of water with Transmission Electron Microscopy (TEM).

Poseidon Sample Prep
Sample Preparation	Sample preparation is simplified in this step by step guide of best procedures with the Poseidon liquid in situ system
Whole Mount Cells	Whole mount cells can be easily prepared using the streamlined process detailed in this note.